Nitrate reductase clone and expression
WebbThe nitrate reductase of fungi and plants is a soluble (or easily solubilized) NADPH-dependent assimilatory enzyme. Bacteria possess either a soluble assimilatory enzyme … Webb12 maj 2011 · Energy supply by a carbon source seems to be the major regulator of nitrate reductase gene expression for fungi ... soils for >50% of the clones. Fungal euknr gene expression in ...
Nitrate reductase clone and expression
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Webb29 mars 2003 · Further work has led to the selection of nitrate reductase-deficient mutants (Marmeisse et al. 1998) and the cloning of a functional nitrate reductase encoding gene (nar1; Jargeat et al. 2000). Transcription of this gene is strongly but not fully repressed by ammonium and, surprisingly, does not need nitrate for induction, as … Webb1 juni 1992 · cDNA Clones for Corn Leaf NADH:Nitrate Reductase and Chloroplast NAD (P):Glyceraldehyde-3-Phosphate Dehydrogenase : Characterization of the Clones and Analysis of the Expression of the Genes in Leaves as Influenced by Nitrate in the Light and Dark. Gowri G , Campbell WH Plant Physiol, (3):792-798 1989 MED: 16666879
Webb1 mars 2007 · A cDNA corresponding to the nitrate reductase (NR) gene from Dunaliella salina was isolated by RT-PCR and (5′/3′)-RACE techniques. The full … Webb13 apr. 2024 · Our study shows that expression of NPF8.4 and most ... glycolate oxidase; HPR1, hydroxypyruvate reductase 1; 2OG, 2 ... Table 4), and cloned into the oocyte expression vector ...
Webb14 apr. 2024 · Low-light intensity affects plant growth and development and, finally, causes a decrease in yield and quality. There is a need for improved cropping strategies to solve the problem. We previously demonstrated that moderate ammonium:nitrate ratio (NH4+:NO3−) mitigated the adverse effect caused by low-light stress, although … WebbThree tobacco nitrite reductase (NiR) cDNA clones were isolated using spinach NiR cDNA as a probe. Sequence analysis and Southern blot hybridization revealed four …
Webb15 juni 2015 · The nitrate reductase gene (nir), encoding the nitrite reductase enzyme (NiR) in Lactobacillus plantarum DMDL 9010, was cloned, and the expressed product was purified. The nir fragment was...
Nitrate reductase (NR) is a key enzyme in nitrogen (N) acquisition by plants, algae and fungi. NR can catalyze NAD(P)H reduction of nitrate to nitrite, which is a rate-limiting step for N acquisition in organisms . shorty kellamWebbAbstract. Nitrate reductase (NR) is a key enzyme that catalyzes the first step in plants nitrogen metabolization. A cDNA of a NR gene from non-heading Chinese cabbage (Brassica campestris ssp. chinensis Makino) cultivar ‘Suzhouqing’ was isolated by RT-PCR and (5′/3′)-RACE techniques. The full-length cDNA sequence of 3049 bp contained an ... sarah has optimal health but she livesWebbNitrate reductase is a key enzyme in nitrogen assimilation, and it catalyzes the nitrate-to-nitrite reduction process in plants. A variety of factors, including nitrate, light, … sarah has optimal healthWebb1 jan. 1999 · NADH-Cyt b5 reductase is a membrane-bound flavoprotein containing a single FAD as a prosthetic group. It transfers electrons from NADH to Cyt b5, which is another membrane protein containing a single heme group. sarah has shown and goal attributesWebbThe accumulation of both leaf and root NiR mRNAs was induced by nitrate and repressed by nitrate- or ammonium-derived metabolites. In addition, the expression of the root … sarah has a jacket with a fur collarWebb14 apr. 2024 · The gene expression encoding nitrate reductase (NR) in leaf blades was significantly enhanced by AWD treatment, whereas nitrite reductase (NIR) remained … sarah hatton willow weaverWebbAll reductive branches of this cycle involve the conversion of nitrate to nitrite, which is catalyzed by the enzyme nitrate reductase. The characterization of nitrate reductases … sarah hauser voice actor